CORE A: PROTEOMICS


Director, Robert N. Cole, Ph.D.
rcole@jhmi.edu
410-614-6968


Associate Director, Sandra Gabelli, Ph.D.
gabelli@jhmi.edu
410-614-4145

Proteomics Specialist, Tatiana N. Boronina, Ph.D.
tboroni1@jhmi.edu
410-614-6968


Bioinformatician, C. Conover Talbot
cct@jhmi.edu
410-614-0443

GOALS

The Proteomics Core applies cutting edge mass spectrometry technology to identify, quantify and characterize proteins or their modifications for assisting Center Members in elucidating the molecular mechanisms mediating normal and pathophysiology in the small intestine, colon, liver/biliary and pancreas.

SERVICES

1. Free Consultation, pre- and post-analysis
2. Sample Preparation: Buffer exchange; Column Chromatography; Proteolytic digestion; Stable isotope labeling
3. Protein Identification of proteins in solution, in gel bands or spots, or in complex protein mixtures
4. Protein Modifications: acetylation, AMPylation, citrullination, glycosylation, phosphorylation, proline hydroxylation, oxidation, ubiquitination, and novel cleavage sites and more.
5. Protein Quantification: Isobaric mass tagging with Tandem Mass Tags (TMT), Absolute Quantification (AQUA), Protein Standard Absolute Quantification (PSAQ), Triggered by Offset, Multiplexed, Accurate-mass, High-resolution, and Absolute Quantification (TOMAHAQ)
6. High-Resolution Mass Analysis of Intact Proteins: protein characterization and their modifications
7. Biostatical/Bioinformatic analysis: Database searches, Data analysis, PCA analysis, Volcano plots, Ingenuity based gene ontology pathway analysis; bioinformatics consultation.

WEBSITES

For additional information about our services and equipment, please go to our website or our iLabs website.