Understand several areas of GI physiology and pathophysiology related to regulation of epithelial function, especially via changes in signal transduction and trafficking, role of neurogastroetnerology, inflammation and injury leading to fibrosis and pre-cancer.
Regulation of epithelial function, especially via changes in signal transduction, trafficking and development.

Center Rationale
This Center is based on the view that one of the hallmarks of science at Johns Hopkins is the interest of basic and clinical investigators in interacting to allow the expertise of both groups to benefit the scientific inquiry into the causes and treatments of basic biologic processes and the diseases which occur when these processes become abnormal. The Research Base consists of 53 investigators, mostly at the Johns Hopkins University School of Medicine. The themes of the Research Base have been similar to that emphasized over the past 8 years of the Development Core Center and include:
1) Epithelial Transporter Function and Regulation, including trafficking, protein-protein interactions and bioinformatics. Major diseases studied: diarrhea, Wilson’s disease, Menke’s disease, cystic fibrosis.
2) Inflammation, injury and fibrosis. Major diseases studied: infectious diarrheal diseases; inflammatory bowel diseases; inflammatory bowel diseases; cirrhosis; Nxxx, eos esophagitis,

necrotizing enteritis, enterotoxigenic B. fragilis; fatty liver, alcoholic and non-alcoholic steatohepatitis, obesity; eosinophilic esophagitis, pancreatitis.
3) Neurogastroenterology. Major diseases studied: IBS, IBD, diarrhea, pain in GI disease.
4) ) GI pre-cancer. Major diseases studied: inflammation, epigenetics and biomarkers, cancer of colon, pancreas and liver.

The Cores provide cutting edge techniques that generally involve technology that is too expensive to be developed and maintained by individual laboratories. These include the Proteomics and Imaging Cores and components of the Integrated Physiology Core (human enteroids, Ussing chamber/voltage clamp and cytokine multiplex assays). In addition, the Cores provide high quality routine services, including help with use of metabolic cages, histology preparation, genotyping. The Translational Research Enhancement Core is a centerpiece of the Johns Hopkins School of Medicine program to advance translational studies in disease.